Diagnose whooping cough based on the clinical and epidemiological data, as well as with the help of laboratory tests, which include several types of analyzes:

- bacteriological method,
- serological methods,
- enzyme immunoassay method,
- molecular method,
- Hematologymethod,
- immunofluorescent study and latex microagglutination (refer to the rapid diagnosis). According to leading experts, now there are all possibilities for the laboratory diagnosis of pertussis infection in almost any stage and irrespective of the form of the disease.

bacteriological method



To conduct this study, patients take a sample of mucus from the back of the throat.A precipitate is taken for analysis or fasting, or 2-3 hours after the last meal.Curren
tly, there are two basic ways to fence mucus - "cough plate" and "zadneglotochny swab."With this method, the research laboratory of pre-trial detention will be able to for 3-5 days.For the final result may need 5-7 days.This method is used for diagnostic examinations of persons coughing over 7 days.The percentage of bacteriologically confirmed cases of pertussis is usually 15-25%. is highly specific molecular diagnostic method for pertussis, now it is widely used abroad and only beginning to take root in Russia.

Serological and ELISA studies



Such tests as TPHA, RA, Phragmites, are commonly used to diagnose the disease in the later stages, as well as epidemiological studies, when experts identify possible foci of infection.By using enzyme immunoassay (EIA) in the early stages of experts determine the content of antibodies of class Ig M, and in the later stages of the disease - Ig G.

Express methods of diagnosis of pertussis



These methods have been established on the basis of intensive research.Using immunofluorescence techniques (RNIF) in the laryngopharyngeal washout from the back of the throat can be identified corpuscular antigens of B. pertussis have for 2-6 hours, which makes it possible to quickly enough to confirm the diagnosis, as well as to carry out the differential diagnosis of symptoms characteristic forother diseases.During the microagglutination of latex (LMA) to identify the presence of mucus in the posterior pharyngeal wall pertussis antigens can for 30-40 minutes.

Hematology method



In this case, patients need to donate blood.In the laboratory, usually revealed leukocytosis with lymphocytosis, while ESR remained normal.This kind of diagnosis of the most indicative for unvaccinated children.